If you read the [url=http://manual.b2evolution.net/Main_Page]docs[/url] you may have noticed the recommendation to rename the sample.htaccess file to .htaccess on an Apache server.
I did rename the file a long time ago, but forgot all about it during the upgrades I did. That's a pity. With the .htaccess in the root folder of the blog, it may load seconds faster. Who doesn't want that?

Good luck